SRPK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87049

Novus Biologicals, part of Bio-Techne
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NBP1-87049
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SRPK2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRPK2 Antibody

Western Blot: SRPK2 Antibody [NBP1-87049]

Western Blot: SRPK2 Antibody [NBP1-87049]

Western Blot: SRPK2 Antibody [NBP1-87049] - Analysis in control (vector only transfected HEK293T lysate) and SRPK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SRPK2 Antibody [NBP1-87049]

Immunocytochemistry/ Immunofluorescence: SRPK2 Antibody [NBP1-87049]

Immunocytochemistry/Immunofluorescence: SRPK2 Antibody [NBP1-87049] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Immunohistochemistry-Paraffin: SRPK2 Antibody [NBP1-87049]

Immunohistochemistry-Paraffin: SRPK2 Antibody [NBP1-87049]

Immunohistochemistry-Paraffin: SRPK2 Antibody [NBP1-87049] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Applications for SRPK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRPK2

Serine/arginine rich protein-specific kinase 2 (SRPK2) phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. SRPK2 plays a role in spliceosome assembly and in mediating the trafficking of splicing factors. SRPK2 also appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Long Name

SFRS Protein Kinase 2

Alternate Names

SFRSK2

Gene Symbol

SRPK2

Additional SRPK2 Products

Product Documents for SRPK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SRPK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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