SRPR alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66583

Novus Biologicals, part of Bio-Techne
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NBP2-66583
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Key Product Details

Species Reactivity

Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SRPR alpha Antibodies »

Product Specifications

Immunogen

The antibody to SRPR alpha was raised against a recombinant protein corresponding to amino acids 39-295 which includes part of the amino terminal SRPR beta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for SRPR alpha Antibody

Western Blot: SRPR alpha Antibody [NBP2-66583]

Western Blot: SRPR alpha Antibody [NBP2-66583]

Western Blot: SRPR alpha Antibody [NBP2-66583]

Applications for SRPR alpha Antibody

Application
Recommended Usage

Western Blot

1-5 ug/ml
Application Notes
Also can be used to immunoprecipitate both subunits of SRP receptors under non-denaturing conditions. Optimal concentration should be evaluated by serial dilutions..

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRPR alpha

The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.

Alternate Names

Docking protein alpha, DP, DP-alpha, MGC17355, MGC3650, MGC9571, signal recognition particle receptor (docking protein), signal recognition particle receptor ('docking protein'), signal recognition particle receptor subunit alpha, Sralpha, SR-alpha, SRP-alpha

Gene Symbol

SRPRA

UniProt

P06625 (Canine)

Additional SRPR alpha Products

Product Documents for SRPR alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SRPR alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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