SSEA-1 Antibody (FR4A5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11605

Novus Biologicals, part of Bio-Techne
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NBP3-11605
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # FR4A5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (FR4A5) - Azide and BSA Free

Immunogen

Myelomonocytic leukemia cells

Localization

Cell surface and granular paranuclear

Marker

Reed-Sternberg Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45304).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for SSEA-1 Antibody (FR4A5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (FR4A5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SSEA-1 Antibody (FR4A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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