SSEA-1 Antibody (SPM490)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45305

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # SPM490

Concentration

0.2 mg/ml

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (SPM490)

Immunogen

Myelomonocytic leukemia cells

Localization

Cell surface and granular paranuclear

Marker

Reed-Sternberg Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11606)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SSEA-1 Antibody (SPM490)

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (SPM490) [NBP2-45305]

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (SPM490) [NBP2-45305]

Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (SPM490) [NBP2-45305] - ICC/IF staining of U937 cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: SSEA-1 Antibody (SPM490) [NBP2-45305]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (SPM490) [NBP2-45305]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (SPM490) [NBP2-45305] - Human Hodgkin's Lymphoma stained with CD15 Monoclonal Antibody (SPM490)

Applications for SSEA-1 Antibody (SPM490)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein L or PEG purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

UniProt

P51993, P22083 (Human)

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (SPM490)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSEA-1 Antibody (SPM490)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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