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SSH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23410

Novus Biologicals, part of Bio-Techne
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NBP3-23410

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1. This sequence has 100% homology with similar regions of mouse and bovine SSH1, and has low homology to SSH2L and SSH3L. [UniProt# Q8WYL5]

Specificity

The antibody detects a 150 kDa protein corresponding to the molecular mass of SSH1 on SDS-PAGE immunoblots of PC12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SSH1 Antibody

SSH1 Antibody

Western Blot: SSH1 Antibody [NBP3-23410] -

Western Blot: SSH1 Antibody [NBP3-23410] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).

Applications for SSH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SSH1

SSH1, also known as slingshot, is a phosphatase that functions to activate the actin severing protein, ADF/cofilin, and influence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3. SSH1 has been shown to function downstream of alpha6beta4 integrin/Rac1 signaling in conjunction with 14-3-3 association.

Alternate Names

EC 3.1.3.16, EC 3.1.3.48, FLJ21928, hSSH-1L, KIAA1298FLJ38102, protein phosphatase Slingshot homolog 1, slingshot 1, slingshot homolog 1 (Drosophila), SSH1L, SSH-1L, SSH-like protein 1

Gene Symbol

SSH1

Additional SSH1 Products

Product Documents for SSH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SSH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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