SSX4 Antibody (3E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00006759-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SSX4 Antibodies »

Product Specifications

Immunogen

SSX4 (NP_005627, 91 a.a. - 188 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNQVERPQMTFGSLQRIFPKIMPKKPAEEENGLKEVPEASGPQNDGKQLCPPGNPSTLEKINKTSGPKRGKHAWTHRLRERKQLVVYEEISDPEEDDE

Specificity

SSX4 - synovial sarcoma, X breakpoint 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SSX4 Antibody (3E10)

Western Blot: SSX4 Antibody (3E10) [H00006759-M02]

Western Blot: SSX4 Antibody (3E10) [H00006759-M02]

Western Blot: SSX4 Antibody (3E10) [H00006759-M02] - Western Blot detection against Immunogen (36.52 KDa) .
Immunocytochemistry/ Immunofluorescence: SSX4 Antibody (3E10) [H00006759-M02]

Immunocytochemistry/ Immunofluorescence: SSX4 Antibody (3E10) [H00006759-M02]

Immunocytochemistry/Immunofluorescence: SSX4 Antibody (3E10) [H00006759-M02] - Analysis of monoclonal antibody to SSX4 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: SSX4 Antibody (3E10) [H00006759-M02] - Detection limit for recombinant GST tagged SSX4 is approximately 3ng/ml as a capture antibody.
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Applications for SSX4 Antibody (3E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SSX4

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

Cancer/testis antigen 5.4, CT5.4SSX4B, MGC119056, MGC12411, protein SSX4, SSX4A, synovial sarcoma, X breakpoint 4

Entrez Gene IDs

6759 (Human)

Gene Symbol

SSX4

OMIM

300326 (Human)

UniProt

NP_005627 (Human)

Additional SSX4 Products

Product Documents for SSX4 Antibody (3E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSX4 Antibody (3E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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