ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33719

Novus Biologicals, part of Bio-Techne
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NBP2-33719
NBP2-33719-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SVIQRAFEDLVNATWREKLLQRLHSLNGSILWIPAFMARGGKERVEWVNE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Western Blot: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Western Blot: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Western Blot: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Immunocytochemistry/ Immunofluorescence: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Immunocytochemistry/Immunofluorescence: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719] - Immunofluorescent staining of human cell line RH-30 shows localization to microtubule organizing center.
Immunohistochemistry-Paraffin: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Immunohistochemistry-Paraffin: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719]

Immunohistochemistry-Paraffin: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [NBP2-33719] - Staining of human heart muscle shows cytoplsmic positivity in myocytes.

Applications for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2

ST8SIA2 is encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2

Alternate Names

SIAT8B, ST8SiaII, STX

Gene Symbol

ST8SIA2

UniProt

Q92186

Additional ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Products

Product Documents for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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