Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all STAG2 Antibodies »

Product Specifications

Immunogen

A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded in part by exon 31 and 32 (LocusLink ID 10735).

Specificity

NB100-301 is specific for human SA2 protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for STAG2 Antibody

Western Blot: STAG2 Antibody [NB100-301]

Western Blot: STAG2 Antibody [NB100-301]

Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301]

Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301]

Immunocytochemistry/Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Immunoprecipitation: STAG2 Antibody [NB100-301]

Immunoprecipitation: STAG2 Antibody [NB100-301]

Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 antibody used for IP at 6 ug per reaction. SA2 was also immunoprecipitated by rabbit anti-SA2 antibodies. For blotting immunoprecipitated SA2,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for STAG2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunoprecipitation

2 - 10 ug/mg of lysate

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Gene Symbol

STAG2

Additional STAG2 Products

Product Documents for STAG2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for STAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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