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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # STAR/2140

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment (around aa 39-108) of human StAR protein (exact sequence is proprietary) (Uniprot: P49675)

Localization

Cytoplasmic (Mitochondria)

Specificity

Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.

Marker

Leydig Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for StAR Antibody (STAR/2140) [CoraFluor™ 1]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: StAR

StAR is encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Alternate Names

cholesterol trafficker, mitochondrial steroid acute regulatory protein, StAR, StARD1, STARD1StAR-related lipid transfer (START) domain containing 1, START domain containing 1, START domain-containing protein 1, steroid acute regulatory protein, steroidogenic acute regulator, steroidogenic acute regulatory protein, steroidogenic acute regulatory protein, mitochondrial

Gene Symbol

STAR

Additional StAR Products

Product Documents for StAR Antibody (STAR/2140) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for StAR Antibody (STAR/2140) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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