STARD10 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78818

Novus Biologicals, part of Bio-Techne
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NBP2-78818
NBP2-78818SS
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all STARD10 Antibodies »

Product Specifications

Immunogen

Full length recombinant human STARD10 protein (amino acids 1-291) [UniProt Q9Y365]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STARD10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: STARD10 Antibody - BSA Free [NBP2-78818]

Immunocytochemistry/ Immunofluorescence: STARD10 Antibody - BSA Free [NBP2-78818]

Immunocytochemistry/Immunofluorescence: STARD10 Antibody [NBP2-78818] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STARD10 Antibody (NBP2-78818) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: STARD10 Antibody - BSA Free [NBP2-78818]

Immunohistochemistry-Paraffin: STARD10 Antibody - BSA Free [NBP2-78818]

Immunohistochemistry-Paraffin: STARD10 Antibody [NBP2-78818] - Analysis of a FFPE tissue section of mouse colon using 1:200 dilution of STARD10 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: STARD10 Antibody - BSA Free [NBP2-78818]

Immunocytochemistry/ Immunofluorescence: STARD10 Antibody - BSA Free [NBP2-78818]

Immunocytochemistry/Immunofluorescence: STARD10 Antibody [NBP2-78818] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STARD10 Antibody (NBP2-78818) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Applications for STARD10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 3 ug/ml

Immunohistochemistry

1:200 - 1:400

Immunohistochemistry-Paraffin

1:200 - 1:400

Western Blot

1 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STARD10

STARD10, STAR-related lipid transfer protein 10 or PCTP-like (33 kDa) is a transporter for lipids which specifically shuttles phosphatidylcholine and phosphatidylethanolamine between membranes. Mammalian STARD10 belongs to a family of fifteen START-domain proteins. In these family members, the carboxy terminal START-domain is conserved and functions in lipid binding or as a lipid sensing domain. The specificity for the type of lipid bound differs among family members (e.g., STARD1,3,5 binds Cholesterol, STARD5 binds 25-hydroxycholesterol, STARD2 binds phosphatidylcholine, and STARD11 binds ceramides). STARD proteins have been associated with various cellular processes such as lipid trafficking, lipid metabolism, and cell signaling (1, 2).

STARD10 is broadly expressed in different organs and tissues such as the brain, endocrine tissues, liver, and gastrointestinal tract. STARD10 is regulated by Casein kinase II, which phosphorylates STARD10 at serine 284 leading to decreased lipid transfer activity and reduced membrane association. STARD10 is overexpressed in breast carcinoma cell lines and in primary breast cancers (3). STARD10 has been implicated in the regulation of bile acid homeostasis and more recently in the regulation of insulin secretion from beta-cells (1, 2, 4).

References

1. Alpy, F., & Tomasetto, C. (2005). Give lipids a START: The StAR-related lipid transfer (START) domain in mammals. Journal of Cell Science. https://doi.org/10.1242/jcs.02485

2. Alpy, F., Legueux, F., Tomasetto, C., & Bianchetti, L. (2009). START domain-containing proteins: A review of their role in lipid transport and exchange. Medecine/Sciences. https://doi.org/10.1051/medsci/2009252181

3. Olayioye, M. A., Hoffmann, P., Pomorski, T., Armes, J., Simpson, R. J., Kemp, B. E., ... Visvader, J. E. (2004). The phosphoprotein StarD10 is overexpressed in breast cancer and cooperates with ErbB receptors in cellular transformation. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-03-3731

4. Carrat, G. R., Hu, M., Nguyen-Tu, M. S., Chabosseau, P., Gaulton, K. J., van de Bunt, M., ... Rutter, G. A. (2017). Decreased STARD10 Expression Is Associated with Defective Insulin Secretion in Humans and Mice. American Journal of Human Genetics. https://doi.org/10.1016/j.ajhg.2017.01.011

Long Name

StAR Related Lipid Transfer Domain Containing 10

Alternate Names

CGI-52, NY-CO-28, PCTP-L, PCTP2, SDCCAG28

Gene Symbol

STARD10

Additional STARD10 Products

Product Documents for STARD10 Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for STARD10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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