Stathmin 1 Antibody (STMN1/8023R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20359

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # STMN1/8023R

Concentration

0.2 mg/ml

View all Stathmin/STMN1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Stathmin 1 protein (exact sequence is proprietary)

Localization

Cytoplasm. Cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human brain or melanoma tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Stathmin 1 Antibody (STMN1/8023R)

Stathmin 1 Antibody (STMN1/8023R)

Stathmin 1 Antibody (STMN1/8023R)

Formalin-fixed, paraffin-embedded human tonsil stained with Stathmin 1 antibody (STMN1/8023R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Stathmin 1 Antibody (STMN1/8023R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Stathmin/STMN1

Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with various regulatory functions in cell proliferation, differentiation signaling, and activation (1). In particular, stathmin is involved in the regulation of tubulin dynamics through inhibition of microtubule formation and/or microtubule depolymerization. Stathmin interacts with soulable tubulins (alpha,beta-tubulin) resulting in the formation of T2S complex which sequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulated through phosphorylation at Ser16, Ser25, Ser38 and Ser63 by various protein kinases (e.g. MAP-kinase, p34cdc2 kinase), weakening stathmin's affinity to tubulin (3). A mutation on stathmin can lead to excess build up of mitotic spindle and with possible consequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also known as the generic member of a protein family which includes neural proteins SCG10, SCLIP and RB3/RB3/RB3. All members in this family exhibits tubulin binding ability.

Alternate Names

LAP18, Metablastin, OP18, Phosphoprotein p19, Prosolin, SMN, STMN1

Gene Symbol

STMN1

Additional Stathmin/STMN1 Products

Product Documents for Stathmin 1 Antibody (STMN1/8023R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Stathmin 1 Antibody (STMN1/8023R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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