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STCH Antibody (1H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006782-M02

Novus Biologicals, part of Bio-Techne
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H00006782-M02

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

STCH (NP_008879.3, 375 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDLFQKILVPIQQVLKEGHLEKTEIDEVVLVGGSTRIPRIRQVIQEFFGKDPNTSVDPDLAVVTGVAIQAGIDGGSWPLQVSALEIPNKHLQKTNF

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

STCH - stress 70 protein chaperone, microsome-associated, 60kDa (1H8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for STCH Antibody (1H8)

Western Blot: STCH Antibody (1H8) [H00006782-M02]

Western Blot: STCH Antibody (1H8) [H00006782-M02]

Western Blot: STCH Antibody (1H8) [H00006782-M02] - STCH monoclonal antibody (M02), clone 1H8. Analysis of STCH expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: STCH Antibody (1H8) [H00006782-M02]

Immunocytochemistry/ Immunofluorescence: STCH Antibody (1H8) [H00006782-M02]

Immunocytochemistry/Immunofluorescence: STCH Antibody (1H8) [H00006782-M02] - Analysis of monoclonal antibody to HSPA13 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: STCH Antibody (1H8) [H00006782-M02]

ELISA: STCH Antibody (1H8) [H00006782-M02]

ELISA: STCH Antibody (1H8) [H00006782-M02] - Detection limit for recombinant GST tagged STCH is approximately 1ng/ml as a capture antibody.

Applications for STCH Antibody (1H8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STCH

The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein. [provided by RefSeq]

Alternate Names

heat shock 70 kDa protein 13, heat shock protein 70kDa family, member 13, microsomal stress 70 protein ATPase core, Microsomal stress-70 protein ATPase core, STCHStress-70 protein chaperone microsome-associated 60 kDa protein, stress 70 protein chaperone, microsome-associated, 60kD, stress 70 protein chaperone, microsome-associated, 60kDa

Entrez Gene IDs

6782 (Human)

Gene Symbol

HSPA13

Additional STCH Products

Product Documents for STCH Antibody (1H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STCH Antibody (1H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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