STEP Antibody (23E5) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-202CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB300-202

Alexa Fluor 350

NB300-202AF350

Alexa Fluor 405

NB300-202AF405

Alexa Fluor 488

NB300-202AF488

Alexa Fluor 532

NB300-202AF532

Alexa Fluor 594

NB300-202AF594

Alexa Fluor 647

NB300-202AF647

Alexa Fluor 700

NB300-202AF700

Alexa Fluor 750

NB300-202AF750

Allophycocyanin

NB300-202APC

Biotin

NB300-202B

DyLight 350

NB300-202UV

DyLight 405

NB300-202V

DyLight 488

NB300-202G

DyLight 550

NB300-202R

DyLight 594

NB300-202DL594

DyLight 650

NB300-202C

DyLight 680

NB300-202FR

DyLight 755

NB300-202IR

FITC

NB300-202F

HRP

NB300-202H

Janelia Fluor 525

NB300-202JF525

Janelia Fluor 549

NB300-202JF549

Janelia Fluor 585

NB300-202JF585

Janelia Fluor 635

NB300-202JF635

Janelia Fluor 646

NB300-202JF646

Janelia Fluor 669

NB300-202JF669

mFluor Violet 450 SE

NB300-202MFV450

mFluor Violet 500 SE

NB300-202MFV500

mFluor Violet 610 SE

NB300-202MFV610

PE

NB300-202PE

PerCP

NB300-202PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # 23E5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all STEP Antibodies »

Product Summary for STEP Antibody (23E5) [CoraFluor™ 1]

Immunogen

A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)

Localization

Cytoplasmic

Specificity

NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for STEP Antibody (23E5) [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

PBS

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: STEP

The protein tyrosine phosphatase non-receptor type 5 or striatum-enriched protein-tyrosine phosphatase (STEP) protein is part of a family of protein tyrosine phosphatases enriched within the CNS, and is a key component of the NMDAR complex. It is expressed within dopaminoceptive neurons in the CNS and particularly enriched within the basal ganglia and related structures. STEP61 is the membrane-associated isoform and STEP46 is the cytosolic protein. In response to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleaved by calpain, thereby releasing a smaller isoform, STEP33.

Long Name

Tyrosine-protein phosphatase non-receptor type 5

Alternate Names

PTPN5

Gene Symbol

PTPN5

Additional STEP Products

Product Documents for STEP Antibody (23E5) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for STEP Antibody (23E5) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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