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STK39 Antibody (0D9L5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16104

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16104-100ul
NBP3-16104-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0D9L5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for STK39 Antibody (0D9L5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 401-545 of human STK39 (Q9UEW8). SQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STK39 Antibody (0D9L5)

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104]

Western Blot: STK39 Antibody (0D9L5) [NBP3-16104] - Western blot analysis of extracts of Mouse testis, using STK39 Rabbit mAb (NBP3-16104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104]

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104]

Immunocytochemistry/Immunofluorescence: STK39 Antibody (0D9L5) [NBP3-16104] - Immunofluorescence analysis of NIH-3T3 cells using STK39 Rabbit mAb (NBP3-16104) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104]

Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104]

Immunohistochemistry-Paraffin: STK39 Antibody (0D9L5) [NBP3-16104] - Immunohistochemistry of paraffin-embedded human placenta using STK39 Rabbit mAb (NBP3-16104) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for STK39 Antibody (0D9L5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STK39

STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Alternate Names

DCHTsmall intestine SPAK-like kinase, DKFZp686K05124, EC 2.7.11, EC 2.7.11.1, PASK, proline-alanine-rich STE20-related kinase, serine threonine kinase 39, Serine/threonine-protein kinase 39, SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast), STE20/SPS1 homolog, STE20/SPS1-related proline-alanine-rich protein kinase, Ste20-like protein kinase, Ste-20-related kinase

Gene Symbol

STK39

Additional STK39 Products

Product Documents for STK39 Antibody (0D9L5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STK39 Antibody (0D9L5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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