Stonin-1 Antibody (1F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00011037-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Stonin-1 Antibodies »

Product Specifications

Immunogen

SBLF (NP_006864, 529 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLKSVVVVQGAYVELQAFVNMASLAQRSSYAGSLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLQELESEPVIQVTVG

Specificity

SBLF - stoned B-like factor (1F3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Stonin-1 Antibody (1F3)

Immunocytochemistry/ Immunofluorescence: Stonin-1 Antibody (1F3) [H00011037-M01]

Immunocytochemistry/ Immunofluorescence: Stonin-1 Antibody (1F3) [H00011037-M01]

Immunocytochemistry/Immunofluorescence: Stonin-1 Antibody (1F3) [H00011037-M01] - Analysis of monoclonal antibody to STON1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Stonin-1 Antibody (1F3) [H00011037-M01] - Detection limit for recombinant GST tagged STON1 is 1 ng/ml as a capture antibody.

Applications for Stonin-1 Antibody (1F3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is useful for ELISA. Use in Western blot reported in scientific literature (PMID 24691443)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Stonin-1

Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Co-transcription of this gene and the neighboring downstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Alternate Names

DKFZp781K2462, FLJ95267, MGC149803, MGC149804, SALF, SBLFstoned-b1, STN1, STNB1, stoned B homolog 1, Stoned B-like factor, stonin 1, stonin-1

Entrez Gene IDs

11037 (Human)

Gene Symbol

STON1

UniProt

NP_006864 (Human)

Additional Stonin-1 Products

Product Documents for Stonin-1 Antibody (1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Stonin-1 Antibody (1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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