Strep II Tag Antibody (661)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43719
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 661
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Strep II Tag Antibody (661)
Immunogen
Immunogen Amino acid sequence - ASWSHPQFEKGA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Strep II Tag Antibody (661)
![Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-661-Immunocytochemistry-Immunofluorescence-NBP2-43719-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719]")
Immunocytochemistry/ Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719]
Immunocytochemistry/Immunofluorescence: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep-tag antibody diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.![Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719]](https://resources.bio-techne.com/images/products/Strep-II-Tag-Antibody-661-Immunoprecipitation-NBP2-43719-img0002.jpg "Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719]")
Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719]
Immunoprecipitation: Strep II Tag Antibody (661) [NBP2-43719] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of strep tagged protein expressing 293T cellsB : Control with 2.5 ug of pre-immune mouse IgGC : Immunoprecipitation of strep tagged protein by 2.5 ug of strep tag antibody 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by strep tag antibody diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Western Blot: Strep II Tag Antibody (661) [NBP2-43719] -
Western Blot: Strep II Tag Antibody (661) [NBP2-43719] -Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [661] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for Strep II Tag Antibody (661)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Strep II Tag
Additional Strep II Tag Products
Product Documents for Strep II Tag Antibody (661)
Product Specific Notices for Strep II Tag Antibody (661)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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