Key Product Details

Species Reactivity

Validated:

Bacterial

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1C2

Format

BSA Free

Concentration

1.0 mg/ml

View all Streptavidin Antibodies »

Product Summary for Streptavidin Antibody (1C2) - BSA Free

Immunogen

Recombinant Streptavidin (25-183aa) purified from E. coli

Reactivity Notes

Streptomyces avidinii

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Streptavidin Antibody (1C2) - BSA Free

Western Blot: Streptavidin Antibody (1C2) [NBP1-04345] - Recombinant Streptavidin protein (17kDa) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system
Streptavidin Antibody (1C2) - BSA Free Western Blot: Streptavidin Antibody (1C2) [NBP1-04345]

Western Blot: Streptavidin Antibody (1C2) [NBP1-04345]

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04345 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Streptavidin protein

Applications for Streptavidin Antibody (1C2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Streptavidin

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Streptavidin Antibody (1C2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Streptavidin Antibody (1C2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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