SUB1 Antibody (04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06541

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 04

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUB1 Antibodies »

Product Summary for SUB1 Antibody (04)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SUB1 (Uniprot#: P53999; Met1-Leu127).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SUB1 Antibody (04)

Western Blot: SUB1 Antibody (04) [NBP3-06541]

Western Blot: SUB1 Antibody (04) [NBP3-06541]

Western Blot: SUB1 Antibody (04) [NBP3-06541] - Lane A: NIH-3T3 Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:16 kDa
Immunocytochemistry/ Immunofluorescence: SUB1 Antibody (04) [NBP3-06541]

Immunocytochemistry/ Immunofluorescence: SUB1 Antibody (04) [NBP3-06541]

Immunocytochemistry/Immunofluorescence: SUB1 Antibody (04) [NBP3-06541] - Staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.
Immunoprecipitation: SUB1 Antibody (04) [NBP3-06541]

Immunoprecipitation: SUB1 Antibody (04) [NBP3-06541]

Immunoprecipitation: SUB1 Antibody (04) [NBP3-06541] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-SUB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUB1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa

Applications for SUB1 Antibody (04)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 ul/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUB1

PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability. Alternate names for PC4 include activated RNA polymerase II transcriptional coactivator p15, SUB1 homolog, positive cofactor 4, p14, SUB1, RPO2TC1, and MGC102747.

Alternate Names

activated RNA polymerase II transcription cofactor 4, activated RNA polymerase II transcriptional coactivator p15, MGC102747, p15, PC4p14P15, Positive cofactor 4, RPO2TC1, SUB1 homolog, SUB1 homolog (S. cerevisiae)

Gene Symbol

SUB1

Additional SUB1 Products

Product Documents for SUB1 Antibody (04)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUB1 Antibody (04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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