SUB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38376
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human SUB1 (NP_006704.3).
Sequence:
MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL
Sequence:
MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SUB1 Antibody
Western Blot: SUB1 Antibody [NBP3-38376] -
Western Blot: SUB1 Antibody [NBP3-38376] - Western blot analysis of various lysates using SUB1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: SUB1 Antibody [NBP3-38376] -
Immunocytochemistry/ Immunofluorescence: SUB1 Antibody [NBP3-38376] - Immunofluorescence analysis of A549 cells using SUB1 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.
Immunoprecipitation: SUB1 Antibody [NBP3-38376] -
Immunoprecipitation: SUB1 Antibody [NBP3-38376] - Immunoprecipitation analysis of 150 ug extracts of HL60 cells using 3 ug SUB1 antibody. Western blot was performed from the immunoprecipitate using SUB1 antibody at a dilution of 1:500.Applications for SUB1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SUB1
Alternate Names
activated RNA polymerase II transcription cofactor 4, activated RNA polymerase II transcriptional coactivator p15, MGC102747, p15, PC4p14P15, Positive cofactor 4, RPO2TC1, SUB1 homolog, SUB1 homolog (S. cerevisiae)
Gene Symbol
SUB1
Additional SUB1 Products
Product Documents for SUB1 Antibody
Product Specific Notices for SUB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...