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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 315 and 365 of human Suppressor of G2 Allele of SKP1 using the numbering given in entry NP_001124384.1 (GeneID 10910).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUGT1 Antibody

Western Blot: SUGT1 Antibody [NBP1-49707]

Western Blot: SUGT1 Antibody [NBP1-49707]

Western Blot: SUGT1 Antibody [NBP1-49707] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SUGT1 antibody NBP1-49707 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SUGT1 was also immunoprecipitated by rabbit anti-SUGT1 antibody NBP1-49706, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Applications for SUGT1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SUGT1

This gene is homologous to the yeast gene SGT1, which encodes a protein involved in kinetochore function and required for the G1/S and G2/M transitions. Complementation studies suggest that the human protein has similar functions.

Alternate Names

Protein 40-6-3, putative 40-6-3 protein, Sgt1, SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae), SGT1B protein, suppressor of G2 allele of SKP1 homolog, suppressor of G2 allele of SKP1, S. cerevisiae, homolog of

Gene Symbol

SUGT1

Additional SUGT1 Products

Product Documents for SUGT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SUGT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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