Sulfatase Modifying Factor 1/SUMF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45212

Novus Biologicals, part of Bio-Techne
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NBP1-45212
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Sulfatase Modifying Factor 1/SUMF1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ETLNPKGPPSGKDR corresponding to internal region according to NP_877437.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Sulfatase Modifying Factor 1/SUMF1 Antibody

Western Blot: Sulfatase Modifying Factor 1/SUMF1 Antibody [NBP1-45212]

Western Blot: Sulfatase Modifying Factor 1/SUMF1 Antibody [NBP1-45212]

Western Blot: Sulfatase Modifying Factor 1/SUMF1 Antibody [NBP1-45212] - Analysis of SUMF1 in Mouse Pancreas lysate (35ug protein in RIPA buffer) using NBP1-45212 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Sulfatase Modifying Factor 1/SUMF1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 37-40 kDa band observed in mouse pancreas, mouse eye and human kidney lysates (calculated MW of 40.6 kDa band according to human NP_877437.2 and 40.6 kDa band according to mouse NP_666049.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sulfatase Modifying Factor 1/SUMF1

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Alternate Names

FGE1

Entrez Gene IDs

285362 (Human); 58911 (Mouse); 362409 (Rat)

Gene Symbol

SUMF1

UniProt

NP_877437.2 (Human)

Additional Sulfatase Modifying Factor 1/SUMF1 Products

Product Documents for Sulfatase Modifying Factor 1/SUMF1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sulfatase Modifying Factor 1/SUMF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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