SUMO1 Antibody (5G3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26296
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5G3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SUMO1 Antibody (5G3)
Immunogen
A synthesized peptide derived from Human SUMO1 [UniProt P63165]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SUMO1 Antibody (5G3)
Immunoprecipitation: SUMO1 Antibody (5G3) [NBP3-26296] -
Immunoprecipitation: SUMO1 Antibody (5G3) [NBP3-26296] - Immunoprecipitating SUMO1 in 293T whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26296 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26296 (3ug) + 293T whole cell lysate (500ug).
Lane 3: 293T whole cell lysate (20ug).
Immunohistochemistry: SUMO1 Antibody (5G3) [NBP3-26296] -
Immunohistochemistry: SUMO1 Antibody (5G3) [NBP3-26296] - Image of SUMO1 Antibody (5G3) diluted at 1:92.5 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry: SUMO1 Antibody (5G3) [NBP3-26296] -
Immunohistochemistry: SUMO1 Antibody (5G3) [NBP3-26296] - Image of SUMO1 Antibody (5G3) diluted at 1:92.5 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Applications for SUMO1 Antibody (5G3)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SUMO1
Long Name
Small Ubiquitin-like Modifier 1
Alternate Names
PIC1, Sentrin, SMT3C, SMT3H3, UBL1
Gene Symbol
SUMO1
Additional SUMO1 Products
Product Documents for SUMO1 Antibody (5G3)
Product Specific Notices for SUMO1 Antibody (5G3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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