SUMO1 Antibody (6F8B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15676

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15676-100ul
NBP3-15676-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6F8B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUMO1 Antibodies »

Product Summary for SUMO1 Antibody (6F8B6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-101 of human Sumo 1 (P63165). MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO1 Antibody (6F8B6)

Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676]

Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676]

Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody (6F8B6) [NBP3-15676]

Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody (6F8B6) [NBP3-15676]

Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (6F8B6) [NBP3-15676] - Immunofluorescence analysis of U-2 OS cells using SUMO1 Rabbit mAb (NBP3-15676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SUMO1 Antibody (6F8B6) [NBP3-15676]

Immunohistochemistry-Paraffin: SUMO1 Antibody (6F8B6) [NBP3-15676]

Immunohistochemistry-Paraffin: SUMO1 Antibody (6F8B6) [NBP3-15676] - Immunohistochemistry of paraffin-embedded human breast cancer using SUMO1 Rabbit mAb (NBP3-15676) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for SUMO1 Antibody (6F8B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUMO1

Sumo 1 is a ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUMO 1 is also known to form a complex with RanGAP1.

Long Name

Small Ubiquitin-like Modifier 1

Alternate Names

PIC1, Sentrin, SMT3C, SMT3H3, UBL1

Gene Symbol

SUMO1

Additional SUMO1 Products

Product Documents for SUMO1 Antibody (6F8B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO1 Antibody (6F8B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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