SUMO4 Antibody (7X3V7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16732

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16732-100ul
NBP3-16732-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X3V7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SUMO4 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO4 (Q6EEV6). MANEKPTEEVKTENNNHINLKVAGQDGSVVQFKIKRQTPLSKLMKAYCEPRGLSVKQIRFRFGGQPISGTDKPAQLEMEDEDTIDVFQQPTGGVY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO4 Antibody (7X3V7)

Western Blot: SUMO4 Antibody (7X3V7) [NBP3-16732]

Western Blot: SUMO4 Antibody (7X3V7) [NBP3-16732]

Western Blot: SUMO4 Antibody (7X3V7) [NBP3-16732] - Western blot analysis of extracts of various cell lines, using SUMO4 Rabbit mAb (NBP3-16732) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody (7X3V7) [NBP3-16732]

Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody (7X3V7) [NBP3-16732]

Immunocytochemistry/Immunofluorescence: SUMO4 Antibody (7X3V7) [NBP3-16732] - Immunofluorescence analysis of U-2 OS cells using SUMO4 Rabbit mAb (NBP3-16732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SUMO4 Antibody (7X3V7) [NBP3-16732]

Immunohistochemistry-Paraffin: SUMO4 Antibody (7X3V7) [NBP3-16732]

Immunohistochemistry-Paraffin: SUMO4 Antibody (7X3V7) [NBP3-16732] - Immunohistochemistry of paraffin-embedded mouse kidney using SUMO4 Rabbit mAb (NBP3-16732) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for SUMO4 Antibody (7X3V7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUMO4

SUMO4 is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq]

Long Name

Small Ubiquitin-like Modifier 4

Alternate Names

dJ281H8.4, IDDM5, small ubiquitin-like modifier 4 protein, Small ubiquitin-like protein 4, small ubiquitin-related modifier 4, SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 4 (yeast), SMT3H4, SUMO-4

Gene Symbol

SUMO4

Additional SUMO4 Products

Product Documents for SUMO4 Antibody (7X3V7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO4 Antibody (7X3V7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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