SUN1 Antibody

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of SUN1 a& nesprin-1 mislocalization in MCF7 and U2OS cells 24 h PI with 8 Gy of gamma-rays. 1a-Central slices through nucleus of MCF7 control show SUN1 & nesprin-1 in whole nucleus. 2-Convoluted nucleus of U2OS. 2a-Slice showing interrupted LA/C. 3-Convoluted nucleus of MCF7 containing 2 large MN, several blisters, and small MN bordered by LAC and filled by SUN1. 5-Tweens of MCF7 daughter nuclei connected by a thin anaphase bridge have fragmented SUN1 in the NE (arrows). 6-U2OS nucleus with amplified SUN1 and a clump (arrow) containing SUN1 and nesprin-1. Size bar indicates 5um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/9/4/999), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of A431 cells at 4ug/ml against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.

Simple Western: SUN1 Antibody [NBP1-87396]

Simple Western: SUN1 Antibody [NBP1-87396] - Simple Western lane view shows a specific band for SUN1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SUN1 Antibody [NBP1-87396]

Simple Western: SUN1 Antibody [NBP1-87396] - Electropherogram image(s) of corresponding Simple Western lane view. SUN1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] -

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of nuclear morphology defects in (A) MCF7 & (B) U2OS cell lines 24 h PI with the dose of 8 Gy of gamma-rays. (A) 1—An anaphase bridge linking the separating daughter nuclei, SUN1 (red), nesprin1 (green), 2—Convoluted nucleus with blisters (arrows), LA/C (green), emerin (red). 3—Fragmented nucleus represented by 3 large & numerous small micronuclei. SUN2 (red), nesprin-1 (green). (B) 1— A giant nucleus with small chromatin fragments & micronuclei (MN, arrows), SUN1 (red), nesprin-1 (green). 2—Nucleus with micronuclei, emerin (red), lamin A/C (green), arrows indicate MN containing emerin & heterochromatin (HC), 3—Convoluted nucleus with blisters (arrows), emerin (red), LA/C (green). 4—Fragmented nucleus, emerin (red), LA/C (green). 5—Honeycombed nucleus, emerin (red), LA/C (green), thick arrow shows interrupted NE enabling chromatin to leak out of the nucleus, reinforced layer of LA/C (green) & emerin (red), (thin arrows). Size bar indicates µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32316379), licensed under a CC-BY license. Not internally tested by Novus Biologicals.