SUPT5H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83508

Novus Biologicals, part of Bio-Techne
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NBP1-83508
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SUPT5H Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22864569)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUPT5H Antibody

Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]

Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508]

Immunohistochemistry-Paraffin: SUPT5H Antibody [NBP1-83508] - Staining in human testis and pancreas tissues using anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.
Western Blot: SUPT5H Antibody [NBP1-83508]

Western Blot: SUPT5H Antibody [NBP1-83508]

Western Blot: SUPT5H Antibody [NBP1-83508] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody [NBP1-83508]

Immunocytochemistry/ Immunofluorescence: SUPT5H Antibody [NBP1-83508]

Immunocytochemistry/Immunofluorescence: SUPT5H Antibody [NBP1-83508] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
View 3 more images

Applications for SUPT5H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUPT5H

SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGT

Alternate Names

DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, FLJ34157, SPT5hSPT5, SPT5HTat-cotransactivator 1 protein, suppressor of Ty (S.cerevisiae) 5 homolog, suppressor of Ty 5 homolog (S. cerevisiae), Tat-CT1, Tat-CT1 protein, transcription elongation factor SPT5

Entrez Gene IDs

6829 (Human)

Gene Symbol

SUPT5H

UniProt

O00267 (Human)

Additional SUPT5H Products

Product Documents for SUPT5H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SUPT5H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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