Survivin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56168

Novus Biologicals, part of Bio-Techne
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NB100-56168
NB100-56168SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Predicted:

Bovine (90%), Canine (91%), Feline (91%), Porcine (92%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Survivin Antibodies »

Product Specifications

Immunogen

This Survivin Antibody was developed against full-length recombinant human survivin protein.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22411272).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Survivin Antibody

Western Blot: Survivin Antibody [NB100-56168]

Western Blot: Survivin Antibody [NB100-56168]

Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.
Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Esophageal Cancer at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Hematoxylin-eosin counterstain.
Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168]

Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Prostate Cancer array at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.

Applications for Survivin Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000. Use reported in scientific literature (PMID 22411273)

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Entrez Gene IDs

11799 (Mouse); 64041 (Rat)

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Survivin Antibody

Product Datasheets

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Product Specific Notices for Survivin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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