SUZ12 Antibody (1B10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26295
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1B10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SUZ12 Antibody (1B10)
Immunogen
A synthesized peptide derived from Human SUZ12 [UniProt Q15022]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SUZ12 Antibody (1B10)
Immunoprecipitation: SUZ12 Antibody (1B10) [NBP3-26295] -
Immunoprecipitation: SUZ12 Antibody (1B10) [NBP3-26295] - Immunoprecipitating SUZ12 in K562 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26295 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26295 (2ug) + K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Immunohistochemistry: SUZ12 Antibody (1B10) [NBP3-26295] -
Immunohistochemistry: SUZ12 Antibody (1B10) [NBP3-26295] - Image of SUZ12 Antibody (1B10) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SUZ12 Antibody (1B10) [NBP3-26295] -
Western Blot: SUZ12 Antibody (1B10) [NBP3-26295] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate.All lanes: SUZ12 Antibody at 1:2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 84 kDa
Observed band size: 90 kDa
Applications for SUZ12 Antibody (1B10)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SUZ12
Long Name
Suppressor of Zeste 12 Homolog
Alternate Names
CHET9, JJAZ1
Gene Symbol
SUZ12
Additional SUZ12 Products
Product Documents for SUZ12 Antibody (1B10)
Product Specific Notices for SUZ12 Antibody (1B10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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