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SV40 Large T Antigen Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13381

Novus Biologicals, part of Bio-Techne
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NBP3-13381

Key Product Details

Species Reactivity

Virus

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of SV40 Large T Antigen (SV40 virus (strain 776)). The exact sequence is proprietary.

Reactivity Notes

Simian virus 40

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SV40 Large T Antigen Antibody

Western Blot: SV40 Large T Antigen Antibody [NBP3-13381]

Western Blot: SV40 Large T Antigen Antibody [NBP3-13381]

Western Blot: SV40 Large T Antigen Antibody [NBP3-13381] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antibody (NBP3-13381) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for SV40 Large T Antigen Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SV40 Large T Antigen

SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Alternate Names

Large T antigen, Small T antigen

Additional SV40 Large T Antigen Products

Product Documents for SV40 Large T Antigen Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SV40 Large T Antigen Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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