SV40 T-antigen Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13382
Key Product Details
Species Reactivity
Virus
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of SV40 T-antigen (SV40 virus (strain 776)). The exact sequence is proprietary.
Reactivity Notes
Simian virus 40
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for SV40 T-antigen Antibody
![Western Blot: SV40 T-antigen Antibody [NBP3-13382]](https://resources.bio-techne.com/images/products/SV40-T-antigen-Antibody-Western-Blot-NBP3-13382-img0001.jpg "Western Blot: SV40 T-antigen Antibody [NBP3-13382]")
Western Blot: SV40 T-antigen Antibody [NBP3-13382]
Western Blot: SV40 T-antigen Antibody [NBP3-13382] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antigen antibody (NBP3-13382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382]](https://resources.bio-techne.com/images/products/SV40-T-antigen-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13382-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382]")
Immunocytochemistry/ Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382]
Immunocytochemistry/Immunofluorescence: SV40 T-antigen Antibody [NBP3-13382] - SV40 T-antigen antibody detects SV40 T-antigen protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SV40 T-antigen stained by SV40 T-antigen antibody (NBP3-13382) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.Applications for SV40 T-antigen Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SV40 T-antigen
Additional SV40 T-antigen Products
Product Specific Notices for SV40 T-antigen Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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