SWAP70 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82979

Novus Biologicals, part of Bio-Techne
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NBP1-82979
NBP1-82979-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SWAP70 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLEEAASRAAEEEKKRLQTQVELQARFSTELEREKLIRQQMEEQVAQKSSELEQYLQRVRELEDMYLKLQEALEDERQARQDEETVRKLQAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SWAP70 Antibody

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in human cell lines A-431 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979]

Western Blot: SWAP70 Antibody [NBP1-82979] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SWAP70 Antibody [NBP1-82979]

Immunocytochemistry/ Immunofluorescence: SWAP70 Antibody [NBP1-82979]

Immunocytochemistry/Immunofluorescence: SWAP70 Antibody [NBP1-82979] - Human dendritic cells were pulsed with FITC-labeled zymosan (zymosan-FITC; green in merge)(pink arrowheads) and latex beads (yellow arrowhead) followed by immunostaining for SWAP70 (magenta). Scale bars, 10 um. Image from a verified customer review.
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Applications for SWAP70 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

validated for ICC/IF from a verified customer review.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-82979 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SWAP70

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion

Long Name

Switch-associated Protein 70 kDa

Alternate Names

KIAA0640SWAP-70FLJ39540, SWAP switching B-cell complex 70kDa subunit, switch-associated protein 70

Gene Symbol

SWAP70

Additional SWAP70 Products

Product Documents for SWAP70 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SWAP70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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