Synapsin I [p Ser549] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-744

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Synapsin I Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 conjugated to KLH. Accession # P17599

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25463523)

Modification

p Ser549

Localization

Cell junction; synapse

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Marker

pre-Synaptic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synapsin I [p Ser549] Antibody

Western Blot: Synapsin I [p Ser549] Antibody [NB300-744]

Western Blot: Synapsin I [p Ser549] Antibody [NB300-744]

Western Blot: Synapsin I [p Ser549] Antibody [NB300-744] - Western blot of rat brain lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser549 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambdlambda-Ptase). The blot is identical ot the control except that it was incubated in lambdlambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho Ser549 synapsin I. The immunolabeling is completely eliminated by treatment with lambdlambda-Ptase.
Immunohistochemistry: Synapsin I [p Ser549] Antibody [NB300-744]

Immunohistochemistry: Synapsin I [p Ser549] Antibody [NB300-744]

Immunohistochemistry: Synapsin I [p Ser549] Antibody [NB300-744] - Immunostaining of cultured mouse caudate neurons showing synapsin I when hosphorylated at Ser549.

Applications for Synapsin I [p Ser549] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synapsin I

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.

Alternate Names

SYN1, phospho Synapsin

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat); 491858 (Canine)

Gene Symbol

SYN1

UniProt

P09951 (Rat)

Additional Synapsin I Products

Product Documents for Synapsin I [p Ser549] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Synapsin I [p Ser549] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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