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Synaptophysin Antibody (SP15)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97459

Novus Biologicals, part of Bio-Techne
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NBP1-97459-0.025ml
NBP1-97459-0.05ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # SP15

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin mAb antibodies.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Pre-synaptic marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Synaptophysin Antibody (SP15)

Western Blot: Synaptophysin Antibody (SP15) [NBP1-97459]

Western Blot: Synaptophysin Antibody (SP15) [NBP1-97459]

Western Blot: Synaptophysin Antibody (SP15) [NBP1-97459] - Analysis of rat liver tissue extract, probed with Synaptophysin, mAb (SP15).

Applications for Synaptophysin Antibody (SP15)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:10000
Application Notes
This antibody is useful in Immunohistochemistry and Western blot (Detects a band of ~38kDa).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin is an integral membrane protein involved in neurotransmitter exocytosis. This protein consists of four transmembrane domains, with its N- and C-terminus facing the cytoplasm. Studies have shown Synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles.

Alternate Names

SYP

Gene Symbol

SYP

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody (SP15)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Synaptophysin Antibody (SP15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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