Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08213
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-SYP-4503R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08213-img0002.jpg "Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SYP/4503R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary) (Uniprot: IDP08247)
Localization
Cytoplasmic
Specificity
This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07325).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]
Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213] - Formalin-fixed, paraffin-embedded human brain stained with Synaptophysin Antibody (SYP/4503R).![Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]](https://resources.bio-techne.com/images/products/Synaptophysin-Antibody-SYP-4503R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08213-img0001.jpg "Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]")
Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213]
Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213] - Formalin-fixed, paraffin-embedded human pancreas stained with Synaptophysin Recombinant Rabbit Monoclonal Antibody (SYP/4503R).Applications for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Synaptophysin
Alternate Names
SYP
Gene Symbol
SYP
Additional Synaptophysin Products
Product Documents for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
Product Specific Notices for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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