Synaptopodin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39100
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SYNPO. The immunogen is located within the last 50 amino acids of SYNPO. Amino Acid Squence: SPSRAWSPRAKQAPRPS
Specificity
At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Synaptopodin Antibody - BSA Free
Western Blot: Synaptopodin AntibodyBSA Free [NBP2-39100]
Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100]
Immunohistochemistry: Synaptopodin Antibody [NBP2-39100] - Mouse brain tissue with SYNPO antibody at 5 ug/mL.Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] -
Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - mTOR signaling pathway regulated PKM2 expression & podocyte differentiation.a IHC detection of p-S6 in renal cortex during postnatal development. Scale bar=20 μm. b Representative western immunoblot analysis of p-S6 in renal cortex. c Representative western immunoblot analysis of p-S6 in cultured podocyte during differentiation. d Western immunoblot analysis of p-S6 & PKM2 expression in Tsc1fl/fl & Tsc1−/−-podocytes. e Rapamycin caused a significant reduction in the protein levels of p-S6 & PKM2 in cultured podocyte. f Western immunoblot analysis of nephrin & synaptopodin with 10 nM rapamycin treatment for 14 days. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Synaptopodin Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2.5 ug/mL
Immunohistochemistry-Paraffin
2.5 ug/mL
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Synaptopodin
Additional Synaptopodin Products
Product Documents for Synaptopodin Antibody - BSA Free
Product Specific Notices for Synaptopodin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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