Synaptotagmin 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13408

Novus Biologicals, part of Bio-Techne
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NBP2-13408
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Synaptotagmin 4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: SNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLKSSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQSMT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Synaptotagmin 4 Antibody

Western Blot: Synaptotagmin 4 Antibody [NBP2-13408]

Western Blot: Synaptotagmin 4 Antibody [NBP2-13408]

Western Blot: Synaptotagmin 4 Antibody [NBP2-13408] - Analysis in control (vector only transfected HEK293T lysate) and SYT4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody [NBP2-13408]

Immunocytochemistry/ Immunofluorescence: Synaptotagmin 4 Antibody [NBP2-13408]

Immunocytochemistry/Immunofluorescence: Synaptotagmin 4 Antibody [NBP2-13408] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane & vesicles.
Immunohistochemistry-Paraffin: Synaptotagmin 4 Antibody [NBP2-13408]

Immunohistochemistry-Paraffin: Synaptotagmin 4 Antibody [NBP2-13408]

Immunohistochemistry-Paraffin: Synaptotagmin 4 Antibody [NBP2-13408] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle cells.

Applications for Synaptotagmin 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptotagmin 4

Synaptotagmin 4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis

Alternate Names

HsT1192, KIAA1342sytIV, synaptotagmin 4, synaptotagmin IVsynaptotagmin-4, SytIV

Gene Symbol

SYT4

Additional Synaptotagmin 4 Products

Product Documents for Synaptotagmin 4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Synaptotagmin 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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