SYNE1 Antibody (6A)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22477

Novus Biologicals, part of Bio-Techne
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NBP2-22477
NBP2-22477-0.025mg
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A

Concentration

1 mg/ml

View all SYNE1 Antibodies »

Product Specifications

Immunogen

Recombinant protein containing residues RKLESTLTGLEQSRERQERR

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SYNE1 Antibody (6A)

Western Blot: SYNE1 Antibody (6A) [NBP2-22477]

Western Blot: SYNE1 Antibody (6A) [NBP2-22477]

Western Blot: SYNE1 Antibody (6A) [NBP2-22477] - Analysis of CPG2 is shown using adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells (kindly provided by Elly Nedivi's lab, MIT, Cambridge, MA) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CPG2 at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.

Applications for SYNE1 Antibody (6A)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SYNE1

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity. There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity.

Alternate Names

ARCA1, C6orf98, CPG2, dJ45H2.2, EC 4.2.1.49, EC 4.3.1.3, EDMD4, Enaptin, KIAA0796chromosome 6 open reading frame 98, KIAA1262FLJ41140, KIAA17568B, Myne-1, MYNE1DKFZp781J13156, Myocyte nuclear envelope protein 1, nesprin 1, nesprin-1, Nuclear envelope spectrin repeat protein 1, SCAR8FLJ30878, spectrin repeat containing, nuclear envelope 1, Synaptic nuclear envelope protein 1, synaptic nuclei expressed gene 1, Syne-1, SYNE-1B

Entrez Gene IDs

499010 (Rat)

Gene Symbol

SYNE1

Additional SYNE1 Products

Product Documents for SYNE1 Antibody (6A)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SYNE1 Antibody (6A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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