Syntaxin 1A Antibody (2B2F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15706

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2B2F10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Syntaxin 1A Antibodies »

Product Summary for Syntaxin 1A Antibody (2B2F10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Syntaxin 1A (Q16623). MKDRTQELRTAKDSDDDDDVAVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syntaxin 1A Antibody (2B2F10)

Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Immunohistochemistry: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Immunohistochemistry: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunofluorescence analysis of mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706]

Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Applications for Syntaxin 1A Antibody (2B2F10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntaxin 1A

Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Alternate Names

Brain Syntaxin, HPC-1, p35-1, STX1A, SYN1A

Gene Symbol

STX1A

Additional Syntaxin 1A Products

Product Documents for Syntaxin 1A Antibody (2B2F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Syntaxin 1A Antibody (2B2F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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