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Syntaxin 7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87497

Novus Biologicals, part of Bio-Techne
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NBP1-87497
NBP1-87497-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Syntaxin 7 Antibody

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497]

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Analysis in human lymph node and pancreas tissues. Corresponding Syntaxin 7 RNA-seq data are presented for the same tissues.
Western Blot: Syntaxin 7 Antibody [NBP1-87497]

Western Blot: Syntaxin 7 Antibody [NBP1-87497]

Western Blot: Syntaxin 7 Antibody [NBP1-87497] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody [NBP1-87497]

Immunocytochemistry/ Immunofluorescence: Syntaxin 7 Antibody [NBP1-87497]

Immunocytochemistry/Immunofluorescence: Syntaxin 7 Antibody [NBP1-87497] - Staining of human cell line U-251 MG shows localization to lysosomes.

Applications for Syntaxin 7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100. Syntaxin 7 antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-87497 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin 7

Syntaxin 7 is involved in the ordered fusion of endosomes and lysosomes with the phagosome, where phagocytic cells kill and degrade internalized foreign particles. Syntaxin 7 is found in late endosomes and lysosomes; whereas Syntaxin 12 is localized to the recycling endosome compartment, both are recruited to the phagosome. However, STX12 is acquired earlier before rapidly recycling off the phagosome, whereas STX7 is recruited later and continues to accumulate throughout the phagosome maturation process.

Alternate Names

STX7

Gene Symbol

STX7

Additional Syntaxin 7 Products

Product Documents for Syntaxin 7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Syntaxin 7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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