Syntaxin-BP1 Antibody (S05-4B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15097

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15097-25ul
NBP3-15097-100ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-4B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Syntaxin-BP1 Antibodies »

Product Summary for Syntaxin-BP1 Antibody (S05-4B6)

Immunogen

A synthetic peptide of human Syntaxin-BP1 (Uniprot#: P61764)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Syntaxin-BP1 Antibody (S05-4B6)

Western Blot: Syntaxin-BP1 Antibody (S05-4B6) [NBP3-15097]

Western Blot: Syntaxin-BP1 Antibody (S05-4B6) [NBP3-15097]

Western Blot: Syntaxin-BP1 Antibody (S05-4B6) [NBP3-15097] - Western blot detection of Syntaxin-BP1 in Hela, A549, C6 cell lysates using Syntaxin-BP1 Rabbit mAb (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.
Western Blot Shows Syntaxin-BP1 Specificity Using Knockout Cell Line.

Western Blot Shows Syntaxin-BP1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma parental cell line and Syntaxin-BP1 knockout U-87 MG cell line (KO). Nitrocellulose membrane was probed with Syntaxin-BP1 Antibody (S05-4B6) (Catalog # NBP3-15097) followed by HRP-conjugated secondary antibody. A specific band was detected for Syntaxin-BP1 at approximately 68 kDa (as indicated) in the parental U-87 MG cell line, but is not detectable in knockout U-87 MG cell line. Primary antibody dilution used: 1/2000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Syntaxin-BP1 by Immunoprecipitation.

Detection of Syntaxin-BP1 by Immunoprecipitation.

U-87 MG human glioblastoma/astrocytoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Syntaxin-BP1 Antibody (S05-4B6) (Catalog # NBP3-15097) pre-coupled to Dynabeads Protein A. Immunoprecipitated Syntaxin-BP1 was detected in Western Blot with NBP3-15097 used at 1/2000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
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Applications for Syntaxin-BP1 Antibody (S05-4B6)

Application
Recommended Usage

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syntaxin-BP1

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part to its ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).

Long Name

Syntaxin-binding Protein 1

Alternate Names

EIEE4, p67, rbSec1, STXBP1, SyntaxinBP1, UNC18

Gene Symbol

STXBP1

Additional Syntaxin-BP1 Products

Product Documents for Syntaxin-BP1 Antibody (S05-4B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Syntaxin-BP1 Antibody (S05-4B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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