Syntrophin Antibody (1351)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1294
![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.bio-techne.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0017.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Amphibian, Canine, Chicken
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1351
Concentration
1 mg/ml
Product Specifications
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Epitope
Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Neuromuscular synapse
Specificity
This antibody detects the alpha 1, beta 1 and beta 2 subunits of Syntrophin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Syntrophin Antibody (1351)
![Western Blot: Syntrophin Antibody (1351) [NB600-1294]](https://resources.bio-techne.com/images/products/Syntrophin-Antibody-1351-Western-Blot-NB600-1294-img0016.jpg "Western Blot: Syntrophin Antibody (1351) [NB600-1294]")
Western Blot: Syntrophin Antibody (1351) [NB600-1294]
Western Blot: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin on canine lysate.![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.bio-techne.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0019.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]
Immunocytochemistry/Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin using Syntrophin Monoclonal Antibody (1351) shows staining in Hela Cells. Syntrophin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Syntrophin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.![Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]](https://resources.bio-techne.com/images/products/Syntrophin-Antibody-1351-Immunocytochemistry-Immunofluorescence-NB600-1294-img0018.jpg "Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]")
Immunocytochemistry/ Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294]
Immunocytochemistry/Immunofluorescence: Syntrophin Antibody (1351) [NB600-1294] - Analysis of Syntrophin using Syntrophin Monoclonal Antibody (1351) shows staining in 293Cells. Syntrophin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Syntrophin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Applications for Syntrophin Antibody (1351)
Application
Recommended Usage
Flow Cytometry
0.5 ug / 10^6 cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
0.1 - 1 ug/ml
Application Notes
WB: Detects an approx. 58 kDa protein representing syntrophin from rat and mouse cell extracts.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Syntrophin
Alternate Names
59 kDa dystrophin-associated protein A1 acidic component 1, dJ1187J4.5, LQT12, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, SNT1alpha-1-syntrophin, syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component), syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component), syntrophin-1, TACIP1acidic alpha 1 syntrophin
Gene Symbol
SNTA1
UniProt
Additional Syntrophin Products
Product Documents for Syntrophin Antibody (1351)
Product Specific Notices for Syntrophin Antibody (1351)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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