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SYPL1 Antibody (EP10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59340

Novus Biologicals, part of Bio-Techne
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NBP2-59340-25ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EP10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for SYPL1 Antibody (EP10)

Immunogen

Immunoprecipitate of human brain

Localization

Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Cell Junction , Synapse

Specificity

Detects 38kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SYPL1 Antibody (EP10)

Western Blot: SYPL1 Antibody (EP10) [NBP2-59340]

Western Blot: SYPL1 Antibody (EP10) [NBP2-59340]

Western Blot: SYPL1 Antibody (EP10) [NBP2-59340] - Analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.
Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]

Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]

Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.
Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]

Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340]

Immunohistochemistry: SYPL1 Antibody (EP10) [NBP2-59340] - Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.

Applications for SYPL1 Antibody (EP10)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SYPL1

SYPL1 is a gene that codes for a protein that has two isoforms, with lengths of 259 and 241 amino acids and weights of approximately 29 and 26 kDa respectively. Current research is being done on diseases and disorders related to this gene including neruonitis and alcoholism. SYPL1 has also been shown to have interactions with VAMP2.

Alternate Names

H-SP1, pantophysin, synaptophysin-like 1, synaptophysin-like protein, synaptophysin-like protein 1, SYPL

Gene Symbol

SYPL1

Additional SYPL1 Products

Product Documents for SYPL1 Antibody (EP10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SYPL1 Antibody (EP10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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