T Plastin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32380

Novus Biologicals, part of Bio-Techne
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NBP1-32380
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Chicken (95%), Rat (100%), Rhesus Macaque (100%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all T Plastin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human T Plastin. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (89%).

Localization

Cytoplasm

Specificity

Thsi antibody is specific to the human PLS3 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for T Plastin Antibody

Western Blot: T Plastin Antibody [NBP1-32380]

Western Blot: T Plastin Antibody [NBP1-32380]

Western Blot: T Plastin Antibody [NBP1-32380] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: T Plastin Antibody [NBP1-32380]

Immunocytochemistry/ Immunofluorescence: T Plastin Antibody [NBP1-32380]

Immunocytochemistry/Immunofluorescence: T Plastin Antibody [NBP1-32380] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: T-Plastin protein stained by T-Plastin antibody diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: T Plastin Antibody [NBP1-32380]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP1-32380]

Immunohistochemistry-Paraffin: T Plastin Antibody [NBP1-32380] - Paraffin-embedded mouse cervix. T-Plastin antibody diluted at 1:500.
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Applications for T Plastin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: T Plastin

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

plastin 3, plastin 3 (T isoform), plastin-3, T plastin, T-plastinT fimbrin

Entrez Gene IDs

5358 (Human)

Gene Symbol

PLS3

UniProt

P13797 (Human)

Additional T Plastin Products

Product Documents for T Plastin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for T Plastin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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