TAF12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12965

Novus Biologicals, part of Bio-Techne
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NBP3-12965
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Canine (98%), Chicken (95%), Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TAF12 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAF12. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TAF12 Antibody

Western Blot: TAF12 Antibody [NBP3-12965]

Western Blot: TAF12 Antibody [NBP3-12965]

Western Blot: TAF12 Antibody [NBP3-12965] - TAF12 antibody detects TAF12 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TAF12 antibody (NBP3-12965) diluted at 1:1000.
Immunoprecipitation: TAF12 Antibody [NBP3-12965]

Immunoprecipitation: TAF12 Antibody [NBP3-12965]

Immunoprecipitation: TAF12 Antibody [NBP3-12965] - Immunoprecipitation of TAF12 protein from 293T whole cell extracts using 5 ug of TAF12 antibody (NBP3-12965). Western blot analysis was performed using TAF12 antibody (NBP3-12965). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
TAF12 Antibody

Western Blot: TAF12 Antibody [NBP3-12965] -

Western Blot: TAF12 Antibody [NBP3-12965] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with TAF12 antibody (NBP3-12965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for TAF12 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAF12

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

20kDa, TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF15, TAF2J, TAFII-20/TAFII-15, TAFII20TAFII20/TAFII15, TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD, Transcription initiation factor TFIID 20/15 kDa subunits, transcription initiation factor TFIID subunit 12

Gene Symbol

TAF12

Additional TAF12 Products

Product Documents for TAF12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TAF12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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