TAK1 [p Thr187] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16950

Novus Biologicals, part of Bio-Techne
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NBP3-16950
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TAK1 Antibodies »

Product Summary for TAK1 [p Thr187] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around T187 of human TAK1 (NP_663304.1). HMTNN

Modification

p Thr187

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TAK1 [p Thr187] Antibody - Azide and BSA Free

Western Blot: TAK1 [p Thr187] AntibodyAzide and BSA Free [NBP3-16950]

Western Blot: TAK1 [p Thr187] AntibodyAzide and BSA Free [NBP3-16950]

Western Blot: TAK1 [p Thr187] Antibody [NBP3-16950] - Analysis of extracts of 293T cells, using TAK1 antibody (NBP3-16950) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: TAK1 [p Thr187] AntibodyAzide and BSA Free [NBP3-16950]

Western Blot: TAK1 [p Thr187] AntibodyAzide and BSA Free [NBP3-16950]

Western Blot: TAK1 [p Thr187] Antibody [NBP3-16950] - Analysis of extracts of NIH/3T3 cells, using TAK1 antibody (NBP3-16950) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for TAK1 [p Thr187] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TAK1

TAK1 (or MAP3K7) was shown to participate in regulation of transcription by transforming growth factor beta (TGF beta). TAK1 is stimulated in response to TGF beta and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF beta superfamily members. TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF b activated kinase 1) MAPKKK in TGF b signal transduction. TAB1 induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1. TNF a activated endogenous TAK1, and the kinase negative TAK1 acted as a dominant negative inhibitor against TNF a induced NFkB activation. TAK1 was suggested to act as a regulatory kinase of IKKs.

Long Name

TGF-beta activated kinase 1

Alternate Names

MAP3K7, TGF1a

Gene Symbol

MAP3K7

Additional TAK1 Products

Product Documents for TAK1 [p Thr187] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TAK1 [p Thr187] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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