Talin1 [p Ser425] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23437

Novus Biologicals, part of Bio-Techne
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NBP3-23437
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Talin1 Antibodies »

Product Specifications

Immunogen

Phospho-Talin1 (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin1. This sequence is conserved in rat, mouse, and chicken Talin1, as well as in Talin2 (Ser-428). [UniProt# Q9Y490]

Modification

p Ser425

Specificity

The antibody detects a 240 kDa protein corresponding to the molecular mass of Talin1 on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Talin1 [p Ser425] Antibody

Talin1 [p Ser425] Antibody

Western Blot: Talin1 [p Ser425] Antibody [NBP3-23437] -

Western Blot: Talin1 [p Ser425] Antibody [NBP3-23437] - Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin1 (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin1 (Ser-425) (lanes 3 & 4) antibodies.

Applications for Talin1 [p Ser425] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Talin1

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Alternate Names

KIAA1027, TLN1

Gene Symbol

TLN1

Additional Talin1 Products

Product Documents for Talin1 [p Ser425] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Talin1 [p Ser425] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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