Talin2 Antibody (7D1Y0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15932

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15932-100ul
NBP3-15932-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7D1Y0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Talin2 Antibodies »

Product Summary for Talin2 Antibody (7D1Y0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Talin2 (Q9Y4G6). VLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKAEHGSV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Talin2 Antibody (7D1Y0)

Western Blot: Talin2 Antibody (7D1Y0) [NBP3-15932]

Western Blot: Talin2 Antibody (7D1Y0) [NBP3-15932]

Western Blot: Talin2 Antibody (7D1Y0) [NBP3-15932] - Western blot analysis of extracts of various cell lines, using Talin2 Rabbit mAb (NBP3-15932) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Talin2 Antibody (7D1Y0) [NBP3-15932]

Immunocytochemistry/ Immunofluorescence: Talin2 Antibody (7D1Y0) [NBP3-15932]

Immunocytochemistry/Immunofluorescence: Talin2 Antibody (7D1Y0) [NBP3-15932] - Immunofluorescence analysis of mouse brain cells using Talin2 Rabbit mAb (NBP3-15932) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Talin2 Antibody (7D1Y0) [NBP3-15932]

Immunohistochemistry-Paraffin: Talin2 Antibody (7D1Y0) [NBP3-15932]

Immunohistochemistry-Paraffin: Talin2 Antibody (7D1Y0) [NBP3-15932] - Immunohistochemistry of paraffin-embedded human lung cancer using Talin2 Rabbit mAb (NBP3-15932) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Talin2 Antibody (7D1Y0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Talin2

Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N-terminal FERM domain that binds integrin cytodomains (aa 88-406), followed by an Ala-rich region (aa 861-1193) and a C-terminal actin-binding domain (aa 2321-2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748-1790. Over aa's 977-1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively.

Alternate Names

ILWEQ, TLN2

Gene Symbol

TLN2

Additional Talin2 Products

Product Documents for Talin2 Antibody (7D1Y0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Talin2 Antibody (7D1Y0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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