Tankyrase 1 Antibody (PSH0-51)

Western Blot: Tankyrase 1 Antibody (PSH0-51) [NBP3-33033]

Western Blot: Tankyrase 1 Antibody (PSH0-51) [NBP3-33033] - Western blot analysis of Tankyrase 1 on different lysates with Rabbit anti-Tankyrase 1 antibody (NBP3-33033) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Raji cell lysate
Lane 5: HepG2 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 142 kDa
Observed band size: 150 kDa

Exposure time: 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33033) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.