Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B10

Concentration

1 mg/ml

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Product Specifications

Immunogen

Recombinant full length 441 amino acid version of the shortest human tau isoform. Epitope located in the peptide HVPGGGNKKIETHKLTFREN, amino acids 362-381. [UniProt# P10636]

Specificity

This antibody is expected to bind to all Tau isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48-67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tau Antibody (5B10)

Western Blot: Tau Antibody (5B10) [NBP2-50051]

Western Blot: Tau Antibody (5B10) [NBP2-50051]

Western Blot: Tau Antibody (5B10) [NBP2-50051] - Analysis of tissue lysates using mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight big tau Isoforms.
Immunocytochemistry/ Immunofluorescence: Tau Antibody (5B10) [NBP2-50051]

Immunocytochemistry/ Immunofluorescence: Tau Antibody (5B10) [NBP2-50051]

Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-50051, dilution 1:2,000 in green, and costained with chicken pAb to GFAP, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-50051 stains perikarya, dendrites and axons of neurons, while the GFAP antibody labels astrocytes.
Immunocytochemistry/ Immunofluorescence: Tau Antibody (5B10) [NBP2-50051]

Immunocytochemistry/ Immunofluorescence: Tau Antibody (5B10) [NBP2-50051]

Immunocytochemistry/Immunofluorescence: Tau Antibody (5B10) [NBP2-50051] - E18 rat brain cultures were grown for 10 days, fixed and immunostained with NBP2-50051 in red and chicken antibody to MAP2 (NB300-213, green) and for DNA (blue). NBP2-50051 stains the neuronal perikarya, dendrites and axonal process strongly and does not stain non neuronal cells in these cultures. The MAP2 antibody also stains neurons only, but only the perikarya and dendrites. As a result perikarya and dendrites appears yellow, since they contain both tau and MAP2, while axons appear red as they contain only tau.

Applications for Tau Antibody (5B10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:10000
Application Notes
In Western blot, multiple bands can be seen in the range of 48-67 kDa. Higher molecular weight "big tau" isoforms mostly in peripheral tissues.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tau

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Gene Symbol

MAPT

UniProt

P10636

Additional Tau Products

Product Documents for Tau Antibody (5B10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tau Antibody (5B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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