Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TBC1D10A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 458 to 508 of human EBP50-PDX Interactor of 64 kDa using the numbering given in entry NP_114143.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBC1D10A Antibody

Western Blot: TBC1D10A Antibody [NBP2-36551]

Western Blot: TBC1D10A Antibody [NBP2-36551]

Western Blot: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPI64 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TBC1D10A Antibody [NBP2-36551]

Immunoprecipitation: TBC1D10A Antibody [NBP2-36551]

Immunoprecipitation: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated EPI64, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds

Applications for TBC1D10A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Reviewed Applications

Read 1 review rated 5 using NBP2-36551 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TBC1D10A

Alternate Names

EBP50-PDX interactor of 64 kDa, EPI64, rab27A-GAP-alpha, TBC1 domain family member 10A, TBC1 domain family, member 10A

Gene Symbol

TBC1D10A

UniProt

NP_114143.1 (Human)

Additional TBC1D10A Products

Product Documents for TBC1D10A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TBC1D10A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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